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KMID : 0364820210570020083
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Korean Journal of Microbiology
2021 Volume.57 No. 2 p.83 ~ p.90
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Characterization and expression of SabA and BabA genes in Helicobacter pylori under varying pH
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Salman Hamzah Abdulrahman
Nayi Eman Mobder
Bloh Anmar Hameed
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Abstract
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Helicobacter pylori is a potential cause for peptic ulcers whichcan make persistent chronic infection. Helicobacter pylori produceurease at low pH to neutralize the acidic environment to colonizethe gastric mucosa. This investigation aimed to characterize H.
pylori based on urease, adherence, motility, and biofilm activityand to determine the gene expression of SabA and BabA byreal-time qRT-PCR. The reference culture H. pylori ATCC49503 was employed in the current study. The characterization ofH. pylori, such as biofilm, urease, adherence, and motility assays,was determined in acidic environments with the supplement ofurea substrate. Real-time qRT-PCR was executed to find thepossible explanation for the colonization by the genes actin,SabA, and BabA. The study indicated that urea substrate isimportant in biofilm activity, adherence, motility, and urease ofH. pylori. Helicobacter pylori ATCC 49503 showed enhancedactivity at pH 2.5 only when supplemented with urea substrate.
Real-time qRT-PCR confirmed the positive and significantexpression of the SabA and BabA genes in an acidic environmentand its cooperative role in biofilm and the motility of H. pylori.
The results propose that urease within H. pylori is necessary toneutralize the acidic niche and colonize effectively within themucosal layers of the stomach. Additionally, the colonizationand adaptability of H. pylori in the in vitro were dependant onurease and pH. Further studies are proposed to understand thecolonization of clinical strains of H. pylori.
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KEYWORD
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BabA, H. pylori, SabA, gene expression, real-time qRT-PCR, urease activity
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